Characterization and heterologous production of a novel laccase from Melanocarpus albomyces

Characterization and heterologous production of a novel laccase from Melanocarpus albomyces by Laura-Leena Kiiskinen Download PDF EPUB FB2

The production levels reported for the expression of Melanocarpus albomyces laccase in T. reesei are the highest heterologous laccase expression levels reported so far, allowing the obtainment of mg l −1 in shake-flask cultures, mg l −1 in batch fermentations, and mg l −1 in fed-batch fermentation.

Actually, a series of. Characterization and kinetic properties of the purified Trematosphaeria mangrovei laccase laccase of Melanocarpus albomyces has a half-life time of 5 h [43].

The thermal stability. lizing laccase. Fur thermore, heterologous expre ssion has been often used a s a strateg y to get around t he problem of obtaining lacca se isoforms not easi ly producible in natura l hosts.

Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea by: 2.

The highest reported yields for heterologous proteins expressed in T. reesei are those of foreign fungal proteins that have reached gram levels, up to g/l for the Melanocarpus albomyces cellulase Cel5A (Table ; Haakana et al., ).The second best yields come from heterologous proteins of bacterial origin nearing and exceeding a gram per liter (Table ; Paloheimo et al., Cited by: 6.

Characterization and heterologous production of a novel laccase from Melanocarpus albomyces / Laura-Leena Kiiskinen. [Espoo, Finland]: VTT Technical Research.

pastoris is a popular host for heterologous laccase production, although laccase yields by P. pastoris typically do not exceed 10 U/mL and often require a long production cycle (e.g., ≥10 day) [8,35,36], which might at least partially be accounted for by difficult expression of laccases in P.

by: 6. Therefore, heterologous production in ascomycete hosts is often the approach of choice, but there are still many challenges in producing these enzymes. Factors hampering heterologous expression of basidiomycete genes in ascomycetes include differences in the gene content such as in codon usage bias, and intron structure and by: 2.

Laccases, multicopper oxidoreductases, are mainly produced in white-rot fungi and are considered as ideal green catalysts in industrial and biotechnological applications.

However, the development of laccases is limited due to the slow growth of natural laccase producing strains and the low expression levels of laccases. In this study, we designed three regulation strategies for laccase gene. Kiiskinen LL, Kruus K, Bailey M, Ylosmaki E, Siika-Aho M, Saloheimo M () Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme.

Microbiology (Pt 9)–Author: Sophie A. Comyn, Sophie A. Comyn, Jon K. Magnuson, Jon K. Magnuson. Production and Characterization of Lignocellulose-Degrading Enzymes Influence of Growth Substrate and Free Ferulic Acid on the Production of Feruloyl Esterase by Aureobasidium pullulans Karl Rumbold.

Filamentous fungi are commonly used in the fermentation industry for the large-scale production of proteins – mainly industrial enzymes. Recent advances in fungal genomics and related experimental technologies such as gene arrays and proteomics are rapidly changing the approaches to the development and use of filamentous fungi as hosts for the production of both homologous and Cited by: A Novel Laccase from the Ascomycete Melanocarpus albomyces / Kristiina Kruus, Laura-Leena Kiiskinen, Markku Saloheimo, Nina Hakulinen, Juha Rouvinen, Arja Paananen, Markus Linder and Liisa Viikari ; Glycoside Hydrolase Gene Cluster of Acidothermus cellulolyticus /.

Melanocarpus albomyces has disulfide bridges inside domain I, another between domain I and domain III, and the last one between domain II and III (Hakulinen et al., ). Laccase has three types of copper atoms present in variations linked with coordinate with each other's and maintain the active site of the amino : Adarsh Kumar, Ram Chandra.

This chapter presents a number of methods that have been successfully applied in the screening for enzymes from extremophilic microorganisms and their communities.

Parameters such as temperature, pH, percentage of relative humidity, salinity, dO2, redox state, moisture content, light, local geology, weather (cloud, precipitation, wind speed), and so on, may be critical or by: 2.

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() Expression and characterization of a novel heterologous moderately thermostable lipase derived from metagenomics in Streptomyces lividans. Journal of Industrial Microbiology and Biotechnology 37(9): Call, H.

P., Mucke, I. () History, overview and applications of mediated. Laccase-catalyzed oxidation of Mn 2+ in the presence of natural Mn 3+ chelators as a novel source of extracellular H 2 O 2 production and its impact on manganese peroxidase.

Appl. Environ. Transgenic raw materials in food production – Detection of transgene and heterologous protein levels. Anna Maria Nuutila/ Anneli Ritala. – Production of recombinant gelatin in barley – an alternative production host.

Anna Maria Nuutila. – A novel approach for the production of pharmaceuticals by plant metabolic. Characterization of the Activity Profile in Cellulases Derived from Recombinant Strains of Trichoderma reesei, F.

Zandona et al. Amplification of Cellulase Genes and Cellulase Hyperproducers in Trichoderma: Minireview, H. Toyama et al. A Novel Laccase from the Ascomycete Melanocarpus albomyces, Kristiina Kruus et al.

Due to its complexity, the degradation of lignocellulosic waste is a great challenge for sustainable development. The generation of lignocellulosic waste from various agro-based industries indicated the magnitude of problems including pulp and paper mill (effluent –m 3 /ton and solid –kg/ton), sugarcane molasses-based distilleries (effluent 15lit/1 lit alcohol production and 7 Author: Adarsh Kumar, Ram Chandra.Preface This book is a part of the comprehensive series Current Developments in Biotechnology and Bioengineering, comprising nine volumes (Editor-in-chief: Ashok Pandey), and deals with the production, isolation, and purification of industrial products produced by biotechnological processes.Cloning of a laccase gene from a novel Basidiomycete Trametes sp.

and its heterologous expression in Pichia pastoris. Curr. A., Hakulinen, N., Structureefunction studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds. J. Heterologous laccase production and its role in industrial.